PCR cloning service: terms and prices

cat.#CS031

You provide

1. Starting materials (cDNA, RNA, genomic DNA, or plasmid DNA)

2. Vector and vector map

3. Sequence information

We perform

PROCEDURES		LEVEL 1 CAT.# CS031-1	LEVEL 2 CAT.# CS031-2
Minimal order size - € 1 000. The prices do not include shipping&handling. We offer DISCOUNTS for MULTIPLE ORDERS!
1. Synthesis of primers designed for PCR amplification
2. cDNA preparation (if required)
3. Amplification of the target fragment
4. Cloning of fragment into the standard pUC-based vector from our collection
5a. Clone confirmation by sequencing in one direction (we will select the clone that contains the target insert, but will not verify the perfect coincidence between the sequence of the insert and the customer provided sequence information)			-
5b. Sequence verification of the perfect coincidence between the sequence of the insert and the customer provided sequence information		-
6. Plasmid purification of a single correct clone (from 5-10 ml culture volume)
Turnaround time		agreed	agreed
Price (per fragment)	0.5-1.5 kb	€ 550	€ 1 150
	1.5-2.5 kb	€ 700	€ 1 700
	2.5-3.5 kb	€ 1 100	€ 2 500
	3.5-4.5 kb	€ 1 900	€ 3 800
	Fragment longer than 4.5 kb	agreed	agreed

Extra options

You will receive

1. Any leftover starting material (on request)

2. PCR primers specific to the target fragment

3. Purified plasmid DNA comprising the target fragment

4. Sequencing data of the clone selected

5. Service report