Full-length-enriched cDNA is usually produced using SMART technology. The method combines cDNA synthesis and amplification and results in representative cDNA population enriched with full-length sequences even from small amounts of starting materials.
After cDNA synthesis, the double stranded cDNA is size fractionated, cloned (directionally or nondirectionally) into a plasmid vector from our collection (or into an appropriate customer-supplied plasmid vector), and transformed into E.coli.
As a quality control measure, a percentage of recombinant clones and average insert size is determined by gel analysis of 33 clones picked at random.