Bacterial genome subtraction
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Bacterial genome subtraction servicecat.# CS022
- Identification of differentially distributed sequences in bacterial strains
- Order processing is set up and monitored by the inventors of the SSH technology
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Supression subtractive hybridization (SSH) is the most powerful non-sequencing approach to find genetic difference between bacterial strains. The ability of SSH to identify nearly all major sequence differences between two closely related bacteria has been demonstrated by several independent research groups (see for example, Akopyants et al., 1998; Agron et al., 2002; Winstanley, 2002; Rebrikov et al., 2002; Rebrikov et al., 2003).
Depending on your particular needs, we offer five levels of custom service. The levels differ in the intensity of work performed and may be finely tuned to exactly suit your research needs. All procedures are set up and monitored by the inventors of the SSH technique.
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 |  | Typical result of differential screening for bacterial SSH-generated libraries.
DNA from the Staphylococcus aureus ZW strain was used as a tester and DNA from the S. aureus 29213 strain was used as a driver in SSH. Differential screening of ZW-specific SSH generated library with unsubtracted ZW and 29213 strain-specific probes (A and B, respectively) showed that this library contains about 95% of differential clones.
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Depending on your particular needs we also offer:
References:
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Agron PG, Macht M, Radnedge L, Skowronski EW, Miller W, Andersen GL.
Use of subtractive hybridization for comprehensive surveys of prokaryotic genome differences.
FEMS Microbiol Lett. 2002; 211 (2):175-82. / pmid: 12076809
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Akopyants NS, Fradkov A, Diatchenko L, Hill JE, Siebert PD, Lukyanov SA, Sverdlov ED, Berg DE.
PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori.
Proc Natl Acad Sci U S A. 1998; 95 (22):13108-13. / pmid: 9789049
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Rebrikov D, Desai S, Kogan YN, Thornton AM, Diatchenko L.
Subtractive cloning: new genes for studying inflammatory disorders.
Ann Periodontol. 2002; 7 (1):17-28. / pmid: 16013213
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Rebrikov DV, Kogan YN, Diatchenko L.
SSH Subtractive Cloning: Comprehensive Survey Of Genome Differences In Prokaryotes.
In: Applications of Genomics and Proteomics for Analysis of Bacterial Biological Warfare Agents. V.G. DelVecchio and V. Krcmery (eds.). IOS Press, Amsterdam , Netherlands. 2003; 352 :95-105.
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Winstanley C.
Spot the difference: applications of subtractive hybridisation to the study of bacterial pathogens.
J Med Microbiol. 2002; 51 (6):459-67. / pmid: 12018652
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