cDNA depletion and library construction service

cat.# CS012

- Specific removal of already analyzed transcripts from cDNA populations
- Preparation of depleted cDNA libraries for functional screenings
- Order processing is set up and monitored by the inventors of the technology

Evrogen offers a highly efficient technology for specific removal of already analyzed transcripts from cDNA populations and preparation of depleted cDNA libraries. Analysis of the resulting depleted cDNA libraries significantly accelerates discovery of unknown genes (Bogdanova et al., 2009). Full-length-enriched cDNA is usually produced using SMART technology. The method combines cDNA synthesis and amplification and results in representative cDNA population enriched with full-length sequences even from small amounts of starting materials.

After cDNA synthesis, the double stranded cDNA is depleted using a Duplex-Specific Nuclease (DSN)- based method. The method allows specific removal of selected transcripts without loss of average cDNA size.

The depleted cDNA is size fractionated, cloned (directionally or nondirectionally) into a plasmid vector from our collection (or into an appropriate customer-supplied plasmid vector), and transformed into E. coli.

Please inquire about other variants of cDNA preparation and special vector requirements.

Depending on your particular needs we also offer the following custom cDNA libraries:

• Standard full-length-enriched cDNA libraries
• Normalized full-length-enriched cDNA libraries
• Subtracted cDNA libraries