cDNA normalization service

cat.# CS010, CS011

- Significant increase in transcriptome sequencing efficiency; especially helpful for high-throughput applications
- Equalization of cDNA population prior to library cloning
- Normalized cDNA suitable for further sequencing by next generation technologies (Roche 454, Illumina Solexa, ABI SOLiD)

Substantial difference in mRNA representation in cells/tissues complicates sequence analysis of primary cDNA libraries, especially for gene discovery projects. Equalization of cDNA population overcomes prevalence of highly abundant transcripts and essentially facilitates discovery of rare genes.

Evrogen duplex-specific nuclease (DSN)-based normalization technology is a highly efficient and well proved approach to equalize transcript abundance in cDNA populations enriched with full-length sequences. High flexibility of the normalization procedure allows easy modifications for various purposes.

Both eukaryotic and prokaryotic RNA can be used as starting material for normalization procedure. Full-length-enriched cDNA is normally produced using modified template-switching (SMART) approach. The method combines cDNA synthesis and amplification and allows generating representative cDNA populations enriched with full-length sequences even from small amounts of starting material. After cDNA synthesis and amplification, double stranded cDNA is normalized using DSN-normalization method.

Related services

• cDNA synthesis and amplification for various applications
• Construction of standard full-length-enriched cDNA libraries
• Construction of subtracted cDNA libraries
• Construction of depleted full-length-enriched cDNA libraries