MOLECULAR BIOLOGY SERVICES:

    cDNA normalization

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cDNA normalization for further 454 sequencing: terms and prices

cat.# CS010, CS011

Starting material

At least 1.0 μg of total RNA or poly(A)+ RNA

Service details

PROCEDURE / CAT.#
CS010 LEVEL 1A
CS011-1 LEVEL 1B
Prices include handling/shipping of service resulting materials (door-to-door delivery to one destination). Shipping of starting materials is paid by sender. We offer DISCOUNTS for MULTIPLE ORDERS!
1a. Synthesis of ds cDNA suitable for further non-directional cloning
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1b. Synthesis of ds cDNA suitable for further directional cloning
-
2. DSN-normalization of cDNA
Turnaround time, weeks
4-6
4-6
Price 2 μg of normalized cDNA provided (standard option)
€ 2 350
€ 2 550
5 μg of normalized cDNA provided
€ 2 700
€ 2 900
7 μg of normalized cDNA provided
€ 2 900
€ 3 100
10 μg of normalized cDNA provided
€ 3 150
€ 3 350
15 μg of normalized cDNA provided
€ 3 600
€ 3 800

Extra options

OPTION DESCRIPTION PRICE
Normalization efficiency analysis Virtual Northern blot is performed for samples from human and mouse with ACTB and UBC genes-derived probes (high abundance in most tissues). For samples from other sources, PCR analysis is used, if sequences of two abundant transcripts are provided by a customer; or Virtual Northern blot is used, if two cloned fragments corresponding to abundant transcripts are provided agreed

Deliverables

1. Amplified non-normalized cDNA (at least 1 μg)

2. Normalized cDNA (at least 2 μg)

3. PCR primers for cDNA amplification

4. Service report

Service pricing request on-line

 

Important Notes

Turnaround time is estimated based on average turnaround time for this type of orders. Actual time may depend on the complexity of starting materials and/or deliverables.

In all the service procedures, outcomes are highly dependent on the nature and quality of starting materials. You will be advised on any concerns we may have related to samples quality after QC tests, and every effort will be made to obtain the best possible results.

Confidentiality statement

All data and materials derived from these services are the physical and intellectual property of the purchaser and will be held strictly confidential by EVROGEN.

Copyright 2002-2010 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com