cDNA subtraction and library construction service

cat.#CS021

- Isolation of differentially expressed genes
- Special approach to eliminate false-positive clones
- Order processing is set up and monitored by the inventors of the SSH technology

cDNA populations are compared using well-known Supression Subtractive Hybridization (SSH) and Mirror Orientation Selection (MOS) technologies. SSH has been used in hundreds of model systems and is now one of the most popular molecular genetic technologies in the world that appropriate for comparison of RNA populations. MOS substantially improves the results of SSH (particular of high complexity cDNA samples) by significant decrease in the number of false-positive clones and increase in the number of differential clones in SSH-generated libraries.

MOS is specially recommended

- for complicated samples (e.g. brain RNA, eukaryotic genomic DNA, etc.);

- for closely related samples;

- if primary SSH PCR requires more than 30 cycles to generate visible PCR products;

- if the percentage of differentially distributed clones in your SSH-subtracted library is very low (i.e. 1-5%);

- if most of the differentially distributed clones found in your SSH-subtracted library are false positives.

Depending on your particular needs, we offer five levels of custom service. The levels differ in the intensity of work performed and may be finely tuned to exactly suit your research project. All procedures are set up and monitored by the inventors of the SSH and MOS tecnologies.

Typical cDNA subtraction and differential screening result. (A) Agarose/EtBr gel-electrophoresis of unsubtracted (1,2); subtracted with SHH (#1 - 1 vs 2; #2 - 2 vs 1) cDNA samples and the samples after MOS procedure; (B, C) Differential screening result of randomly picked clones from #1' sample with radiolabeled subtracted cDNA probes: (B) #1'-probe; (C) #2'-probe.

Depending on your particular needs we also offer the following custom cDNA libraries:

•  Standard full-length-enriched cDNA libraries
  
•  Normalized full-length-enriched cDNA libraries
  
•  Depleted full-length-enriched cDNA libraries