cDNA subtraction and library construction service

cat.# CS021, CS024

- Subtracted cDNA preps suitable for further next generation sequencing
- Isolation of differentially expressed genes
- Special approach to eliminate false-positive clones

cDNA populations are compared using well-known Supression Subtractive Hybridization (SSH) technology. SSH has been used in hundreds of model systems and is now one of the most popular molecular genetic approaches for comparison of RNA populations. Mirror Orientation Selection (MOS) helps to substantially improve SSH results by decreasing the number of false-positive clones and increasing the number of differential clones in SSH-generated libraries.

MOS is specially recommended

- for certain types of samples (e.g. brain RNA, eukaryotic genomic DNA, etc.)
- in the case of small difference between samples
- when primary SSH PCR requires more than 30 cycles to generate visible PCR products
- when percentage of differentially distributed clones in SSH-subtracted library is very low (i.e. 1-5%)
- when most of the differentially distributed clones found in SSH-subtracted library are in fact false positives

The service comes in various levels to meet your project needs and can be performed using eukaryotic or prokaryotic cDNA as a starting material. All procedures are monitored by inventors of SSH and MOS technologies.

Related services

• cDNA synthesis and amplification for various applications
• Construction of standard full-length-enriched cDNA libraries
• Construction of normalized full-length-enriched cDNA libraries
• Construction of depleted full-length-enriched cDNA libraries
• Isolation of full-length cDNA (RACE)