MOLECULAR BIOLOGY SERVICES:

RACE and full-length cDNA isolation service

cat.#CS033

- Isolation of full-length cDNAs when only partial nucleotide or amino acid sequence is known

Evrogen offers isolation of full length cDNA(s) correspondent to a known fragment using Step-Out-RACE technology. This method allows fast isolation of the 3’- and 5’-ends of the target transcript with no background noise.
The method can be successfully applied to total RNA as well as poly(A)+ RNA and is applicable even if only a short nucleotide (20—30 bp) or protein sequence (8—10 aa) is available.

Terms and prices

 

5’- and 3’- Step-Out RACE results.

Amplified cDNA was prepared from human placenta total RNA and used for 5’- and 3’- RACE of several marker genes: IFNGR1 — interferon gamma receptor 1; TFRC — transferrin receptor; HPRT1 — hypoxanthine phosphoribosyltransferase-1; M — 1-kb DNA size markers.


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