Phi-Yellow fluorescent proteins

- Bright yellow fluorescence
- Proven suitability to generate stably transfected cell lines
- Destabilized variant is available

Performance and use

Phi-Yellow proteins can be easily expressed and detected in a wide range of organisms, from bacteria to mammals. Transient transfection of mammalian cell lines with these proteins results in bright yellow fluorescent signals without visible aggregation. Fluorescence is clearly detected within 12 hrs after transfection.

Fluorescent microscopy of transiently transfected mammalian cells expressing Phi-Yellow proteins.

Suitability of Phi-Yellow proteins to generate stably transfected cells has been proven by Marinpharm company. Variuos cell lines are commercially available.

		Fluorescent microscopy of stably transfected mammalian cells expressing PhiYFP in cytosol. (A) M3 mouse melanoma; (B) T-406 human glioma; (C) PC-12 rat phaeochromocytoma cells; (D) PC-12 cells after the addition of nerve growth factor; (E) Walker 256 rat tumour cells; (F) BC3H1 cells; (G) T24 human bladder carcinoma cells; (H) T24 cells expressing destabilized variant PhiYFP-m-dest1. Images were kindly provided by Dr. Christian Petzelt (Marinpharm).

Despite dimerization capacity, Phi-Yellow proteins demonstrate successful performance in fusions with subcellular localization signals and many cellular proteins. However, we recommend that you use TagFPs for protein labeling applications. Please see section "Protein Localization Tags" to select a reporter for such purposes.
Important note: PhiYFP allows generation of fusions to its N-terminus, whereas PhiYFP-m is optimized to generate fusions to its C-terminus. PhiYFP can not be used to generate C-terminal fusions.

Phi-Yellow proteins can be used in multicolor labeling applications with cyan, green, red, and far-red fluorescent dyes.

	Fluorescent microscopy of stably transfected mammalian cells expressing Phi-Yellow-tagged fusions. (A-B) Mitochondria-targeted PhiYFP in (A) 3T3 mouse fibroblasts; (B) PtK rat kangaroo cells; (C) T24 human bladder carcinoma cells expressing peroxisome-targeted PhiYFP-m; (D) PhiYFP-m fusion with β-actin in PtK rat kangaroo cells. Images were kindly provided by Dr. Christian Petzelt (Marinpharm).