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    PhiYFP

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Phi-Yellow fluorescent proteins

- Bright yellow fluorescence
- Proven suitability to generate stably transfected cell lines
- Destabilized variant is available


Available variants and fusions
VariantDescriptionRelated vectorCat.#Click for image
PhiYFP PhiYFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. PhiYFP allows generation of fusions to its N-terminus but unsuited to generate C-terminal fusions. pPhi-Yellow-N FP602
pPhi-Yellow-B FP603
pPhi-Yellow-PRL FP604
PhiYFP-mito fusion A mitochondrial targeting sequence (MTS) is fused to the PhiYFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides green fluorescent labeling of mitochondria. pPhi-Yellow-mito FP607
PhiYFP-m variant PhiYFP-m variant is a mutant of PhiYFP. It is suitable for fusion generation to its C-terminus. pPhi-Yellow-C FP601
Destabilized PhiYFP-m variant (PhiYFP-m-dest1) PhiYFP-m-dest1 is produced by fusing the initial protein with PEST amino acid sequence encoded by region 422-461 of mouse ornithine decarboxylase gene [Li et al., 1998]. This sequence targets the protein to degradation and enables a rapid protein turnover.
PhiYFP-m-dest1 retains spectral properties of the initial protein, but has shorter half-lives (approximately 2 hrs) as measured by the analysis of fluorescence intensity of cells treated with a protein synthesis inhibitor, cycloheximide. Because of rapid turnover, PhiYFP-m-dest1 can be used to measure changes in gene expression. 		pPhi-Yellow-PRL-dest1 FP605
pPhi-Yellow-dest1 FP608
PhiYFP-m-peroxi fusion Peroxisomal targeting signal [Gould et al., 1989] encoding tripeptide SKL was fused to the 3' end of PhiYFP-m sequence. This tripeptide targets the fusion protein to the matrix of peroxisomes. pPhi-Yellow-peroxi FP606


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References:

  • Gould SJ, Keller GA, Hosken N, Wilkinson J, Subramani S. A conserved tripeptide sorts proteins to peroxisomes. J Cell Biol. 1989; 108 (5):1657-64. / pmid: 2654139
  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
  • Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR. Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem. 1998; 273 (52):34970-5. / pmid: 9857028
  • Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
  • Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA. A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues. J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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