Blue fluorescent protein TagBFP

- Bright blue fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high photostability
- Extremely high pH-stability
- Recommended for protein labeling, acidic organelle labeling, FRET applications

Main properties

TagBFP normalized excitation (thin line) and emission (thick line) spectra. Spectra viewer tool Download TagBFP spectra (xls)

CHARACTERISTIC * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Molecular weight, kDa 26 Polypeptide length, aa 233 Fluorescence color blue Excitation maximum, nm 402 Emission maximum, nm 457 Quantum yield 0.63 Extinction coefficient, M-1cm-1 52 000 Brightness* 32.8 Brightness, % of EGFP 99 pKa 2.7 Structure monomer Aggregation no Maturation rate at 37°C fast Photostability high Cell toxicity not observed Main advantages bright blue monomeric fluorescent protein Possible limitations not observed