Blue fluorescent protein TagBFP

- Bright blue fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high photostability
- Extremely high pH-stability
- Recommended for protein labeling, acidic organelle labeling, FRET applications

Available variants and fusions

Variant	Description	Related vector	Cat.#	Click for image
Humanized TagBFP	TagBFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.	pTagBFP-C	FP171
		pTagBFP-N	FP172
TagBFP-AS variant	TagBFP-AS codon usage is optimized for high expression in Arabidopsis and Saccharomyces. This variant is available in Gateway® entry clones.	Gateway® TagBFP-AS-C entry clone	FP177
		Gateway® TagBFP-AS-N entry clone	FP178
TagBFP-actin fusion	Human β-actin is fused to the TagBFP C-terminus. When expressed in mammalian cells, this fusion provides blue fluorescent labeling of β-actin in living cells.	pTagBFP-actin	FP174
TagBFP-tubulin fusion	Human α-tubulin is fused to the TagBFP C-terminus. When expressed in mammalian cells, this fusion provides blue fluorescent labeling of α-tubulin in living cells.	pTagBFP-tubulin	FP175
TagBFP-H2B fusion	Human histone H2B is fused to the TagBFP N-terminus. When expressed in mammalian cells, this fusion provides blue fluorescent labeling of histone H2B in living cells.	pTagBFP-H2B	FP176