Far-red fluorescent protein TagFP635

- Bright far-red fluorescence
- Monomer with successful performance in fusions
- High photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling

Main properties

TagFP635 normalized excitation (thin line) and emission (thick line) spectra. Download TagFP635 spectra (xls)

CHARACTERISTIC *Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Molecular weight, kDa 27 Polypeptide length, aa 237 Fluorescence color far-red Excitation maximum, nm 635 Emission maximum, nm 588 Quantum yield 0.33 Extinction coefficient, M-1cm-1 45000 Brightness* 14.9 Brightness, % of EGFP 45 pKa 6.0 Structure monomer Aggregation no Maturation rate at 37°C fast Photostability high Main advantages monomeric far-red fluorescent protein, high photostability

	Gel-filtration of TurboGFP (dimer, green line), EGFP (monomer, blue line), and TagFP635 (monomer, red line). Image from Shcherbo et al., 2007.