TagGFP normalized excitation (thin line) and emission (thick line) spectra.Download TagGFP spectra (xls) | | CHARACTERISTIC | |
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*Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| | Molecular weight, kDa | 27 | | Polypeptide length, aa | 238 | | Fluorescence color | green | | Excitation maximum, nm | 482 | | Emission maximum, nm | 505 | | Quantum yield | 0.59 | | Extinction coefficient, M-1cm-1 | 58 200 | | Brightness* | 34.3 | | Brightness, % of EGFP | 104 | | pKa | 4.7 | | Structure | monomer | | Aggregation | no | | Maturation rate at 37°C | fast | | Photostability | high | | Cell toxicity | not observed | | Main advantages | bright green monomeric fluorescent protein |
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