Green fluorescent protein TagGFP

- Fast protein maturation
- Bright green fluorescence
- Proven availability to generate stably transfected cell lines
- Monomeric protein with successful performance in fusions
- Recommended for protein labeling

Performance and use

TagGFP can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TagGFP expression vectors give bright fluorescent signals within 10-12 hrs after transfection. No cell toxic effects and visible protein aggregation are observed.

Being expressed in mammalian cells, TagGFP shows brightness and maturation speed similar to those of EGFP. However, compared with EGFP, TagGFP matures more than two times faster in E. coli cells.

TagGFP and EGFP expression in E. coli, 10 hrs after transformation

TagGFP performance in fusions has been demonstrated in the β-actin, α-tubulin and mitochondria-targeting signal models. It can be used in multicolor labeling applications with cyan, yellow, red, and far-red fluorescent dyes.

TagGFP expression in transiently transfected mammalian cells (A) Whole-cell expression; (B) TagGFP fusion with human β-actin; (c) TagGFP fusion with human α-tubulin. See also 3D movie of TagGFP-tagged tubulin

TagGFP suitability to generate stably transfected cells has been proven by Marinpharm company. Cell lines expressing TagGFP fusion with α-tubulin are commercially available from this company.

Stably transfected cell lines expressing TagGFP-tagged fusions (A) TagGFP-tagged α-tubulin in MDCK canine kidney epithelial cells; (B) TagGFP-tagged α-tubulin in T24 cells; (c) TagGFP fusion with human β-actin in U-205 human osteosarcoma cells. Images were kindly provided by Dr. Christian Petzelt (Marinpharm).