Far-red fluorescent protein TurboFP635

- Bright far-red fluorescence
- Fast maturation
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for gene expression analysis, cell and organelle labeling inside of tissues

Performance and use

TurboFP635 can be easily expressed and detected in a wide range of organisms. It can be easy visualised within living tissues. Mammalian cells transiently transfected with TurboFP635 expression vectors give bright fluorescent signals within 10-12 hours after transfection. No cell toxic effects and visible protein aggregation are observed.

		DsRed-Express and TurboFP635 expression in Xenopus laevis. Transgenic 2.5 months living animals expressing TurboFP635 and DsRed-Express under the control of cardiac actin promoter are shown from the dorsal side. TurboFP635 (on the right) is excellently visible in the whole body, while DsRed-Express (on the left) can be hardly visualized. This experiment clearly demonstrates the advantage of longer wavelength emission of TurboFP635 for the whole body imaging. Leica MZFLIII fluorescent stereomicroscope, excitation filter 546/10; emission filter 565LP. Image from Shcherbo et al., 2007.

Despite its dimeric structure, TurboFP635 is suitable for generation of fusions. However, we recommend that you use specially optimized protein localization TagFPs to select a reporter for such purposes.

TurboFP635 suitability to generate stably transfected cells has been proven by Marinpharm company. Variuos cell lines expressing TurboFP635 are commercially available.

TurboFP635 can be used in multicolor labeling applications with other fluorescent proteins from cyan to red (orange) colors.

	TurboFP635 expression in mammalian cells. (A) Transiently transfected Phoenix cells; (B) stably transfected WALKER 256 rat tumor cells; (C) stably transfected T-24 human bladder carcinoma cells; (D) stably transfected metastasizing melanoma line MelJuSo. Images of stably transfected cell lines were kindly provided by Dr. Christian Petzelt (Marinpharm).