TurboRFP


Red fluorescent protein TurboRFP

- Superbright red (orange) fluorescence
- Fast maturation
- Destabilized version is available
- Recommended for gene expression analysis, cell and organelle labeling

TurboRFP is a red fluorescent protein (excitation/emission maxima are 553 and 574 nm, respectively) derived from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. Possessing high photostability and pH stability, TurboRFP is more than twice brighter than DsRed2. Fast TurboRFP maturation makes it clearly detectable in mammalian cells as early as within 8-10 hrs after transfection.
TurboRFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboRFP variant allows accurate analysis of rapid and/or transient events in gene regulation.

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TurboRFP expression/source vectors

pTurboRFP-C FP231 Mammalian expression vector encoding humanazied TurboRFP and allowing its expression and generation of fusions to the TurboRFP C-terminus 20 μg € 400.00
pTurboRFP-N FP232 Mammalian expression vector encoding humanazied TurboRFP and allowing its expression and generation of fusions to the TurboRFP N-terminus 20 μg € 400.00
pTurboRFP-B FP233 Bacterial expression vector; source of the TurboRFP coding sequence 20 μg € 400.00
pTurboRFP-PRL FP235 Promoterless vector encoding humanazied TurboRFP and designed for monitoring transcription from different promoters and promoter/enhancer combinations 20 μg € 400.00
pTurboRFP-mito FP237 Mammalian expression vector encoding humanazied TurboRFP targeted to mitochondria 20 μg € 400.00
pTurboRFP-PRL-dest1 FP238 Promoterless vector encoding destabilized TurboRFP and designed for monitoring transcription from different promoters and promoter/enhancer combinations 20 μg € 400.00
pTurboRFP-dest1 FP239 Mammalian expression vector encoding destabilized TurboRFP for its expression and generation of fusions to the TurboRFP-dest1 N-terminus 20 μg € 400.00


Vector set

Promoter-tracker 3-colors FPP15 Promoterless vectors pTurboYFP-PRL, pTurboGFP-PRL, and pTurboRFP-PRL 20 μg each € 700.00


Antibody against TurboRFP

Anti-tRFP
antibody
AB231 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, TagRFP, and TagFP635 100 μg € 150.00
AB232 200 μg € 200.00

References:

  • Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 2007; 4 (7):555-7. / pmid: 17572680
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