TagRFP normalized excitation (thin line) and emission (thick line) spectra.Download TagRFP spectra (xls) | | CHARACTERISTIC | |
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*Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| | Molecular weight, kDa | 27 | | Polypeptide length, aa | 231 | | Fluorescence color | red (orange) | | Excitation maximum, nm | 555 | | Emission maximum, nm | 583 | | Quantum yield | 0.48 | | Extinction coefficient, M-1cm-1 | 102 000 | | Brightness* | 49.0 | | Brightness, % of EGFP | 148 | | pKa | 3.8 | | Structure | monomer | | Aggregation | no | | Maturation rate at 37°C | fast | | Photostability | medium | | Cell toxicity | not observed | | Main advantages | bright red monomeric fluorescent protein | | Possible limitations | medium photostability |
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