Red fluorescent protein TagRFP

- Bright red (orange) fluorescence
- Fast maturation
- High pH stability
- Monomeric protein with successful performance in fusions
- Recommended for protein labeling

Available variants and fusions

TagRFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.

TagRFP-Golgi fusion: Golgi targeting sequence (fragment of human β-1,4-galactosyltransferase) is fused to the TagRFP N-terminus. When expressed in mammalian cells, this variant provides red fluorescent labeling of Golgi apparatus.

TagRFP-mito fusion: A mitochondrial targeting sequence (MTS) is fused to the TagRFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides red fluorescent labeling of mitochondria.

TagRFP-actinin fusion: Human α-actinin is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of α-actinin in living cells.

TagRFP-tubulin fusion: Human α-tubulin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of α-tubulin in living cells.

TagRFP-integrin fusion: Human α-V-integrin is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of α-V-integrin in living cells.

TagRFP-actin fusion: Human β-actin is linked to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of β-actin in living cells.

TagRFP-FAK fusion: Chicken focal adhesion kinase is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of focal adhesion kinase in living cells.

TagRFP-keratin fusion: Human cytokeratin-18 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of cytokeratin-18 in living cells.

TagRFP-EB3 fusion: Human microtubule-associated EB3 protein is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of EB3 protein in living cells.

TagRFP-profilin fusion: Mouse profilin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of profilin in living cells.

TagRFP-vinculin fusion: Human vinculin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of vinculin in living cells.

TagRFP-zyxin fusion: Human zyxin is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of zyxin in living cells.

TagRFP-Cx26 fusion: Rat connexin 26 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of connexin 26 in living cells.

TagRFP-Cx32 fusion: Human connexin 32 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of connexin 32 in living cells.

TagRFP-Cx43 fusion: Rat connexin 43 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of connexin 43 in living cells.

TagRFP-H2B fusion: Human histone H2B is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of histone H2B in living cells.

TagRFP-laminB1 fusion: Human lamin B1 is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of lamin B1 in living cells.