|
Red fluorescent protein TagRFP
- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications
Available variants and fusions
| Variant | Description | Related vector | Cat.# | Click for image |
|---|
|
|
Humanized TagRFP
|
TagRFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
|
pTagRFP-C
|
FP141
|
|
pTagRFP-N
|
FP142
|
|
TagRFP-AS variant
|
TagRFP-AS codon usage is optimized for high expression in Arabidopsis and Saccharomyces. This variant is available in Gateway® entry clones.
|
Gateway® TagRFP-AS-C entry clone
|
FP148
|
|
Gateway® TagRFP-AS-N entry clone
|
FP149
|
|
TagRFP-actin fusion
|
Human β-actin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of β-actin in living cells.
|
pTagRFP-actin
|
FP144
|
|
|
TagRFP-tubulin fusion
|
Human α-tubulin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of α-tubulin in living cells.
|
pTagRFP-tubulin
|
FP145
|
|
|
TagRFP-mito fusion
|
A mitochondrial targeting sequence (MTS) is fused to the TagRFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of mitochondria.
|
pTagRFP-mito
|
FP147
|
|
|
TagRFP-actinin fusion
|
Human α-actinin is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of α-actinin in living cells.
|
pTagRFP-actinin
|
FP360
|
|
|
TagRFP-integrin fusion
|
Human α-V-integrin is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of α-V-integrin in living cells.
|
pTagRFP-integrin
|
FP361
|
|
|
TagRFP-Cx26 fusion
|
Rat connexin 26 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of connexin 26 in living cells.
|
pTagRFP-Cx26
|
FP362
|
|
|
TagRFP-Cx32 fusion
|
Human connexin 32 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of connexin 32 in living cells.
|
pTagRFP-Cx32
|
FP363
|
|
|
TagRFP-Cx43 fusion
|
Rat connexin 43 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of connexin 43 in living cells.
|
pTagRFP-Cx43
|
FP364
|
|
|
TagRFP-EB3 fusion
|
Human microtubule-associated EB3 protein is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of EB3 protein in living cells.
|
pTagRFP-EB3
|
FP365
|
|
|
TagRFP-FAK fusion
|
Chicken focal adhesion kinase is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of focal adhesion kinase in living cells.
|
pTagRFP-FAK
|
FP366
|
|
|
TagRFP-Golgi fusion
|
Golgi targeting sequence (fragment of human β-1,4-galactosyltransferase) is fused to the TagRFP N-terminus. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of Golgi apparatus.
|
pTagRFP-Golgi
|
FP367
|
|
|
TagRFP-H2B fusion
|
Human histone H2B is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of histone H2B in living cells.
|
pTagRFP-H2B
|
FP368
|
|
|
TagRFP-keratin fusion
|
Human cytokeratin-18 is fused to theTagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of cytokeratin-18 in living cells.
|
pTagRFP-keratin
|
FP369
|
|
|
TagRFP-laminB1 fusion
|
Human lamin B1 is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of lamin B1 in living cells.
|
pTagRFP-laminB1
|
FP370
|
|
|
TagRFP-profilin fusion
|
Mouse profilin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of profilin in living cells.
|
pTagRFP-profilin
|
FP371
|
|
|
TagRFP-vinculin fusion
|
Human vinculin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of vinculin in living cells.
|
pTagRFP-vinculin
|
FP372
|
|
|
TagRFP-zyxin fusion
|
Human zyxin is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of zyxin in living cells.
|
pTagRFP-zyxin
|
FP373
|
|
References:
-
Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
-
Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T.
Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells.
Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
-
Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA.
A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues.
J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
|
