Red fluorescent protein TagRFP

- Bright red (orange) fluorescence
- Fast maturation
- High pH stability
- Monomeric protein with successful performance in fusions
- Recommended for protein labeling

Performance and use

TagRFP can be easily expressed and detected in a wide range of organisms. It becomes clearly detectable in mammalian cells as early as within 10-12 hrs after transfection. No cell toxic effects and visible protein aggregation are observed.

	TagRFP use for cell and organelle labeling. Microscopic images of HeLa cells transiently transfected with TagRFP and TagRFP-targeted to cellular organelles. Images were kindly provided by Michael W. Davidson (Florida State University).

TagRFP performance in protein fusions has been demonstrated in fibrillarin, vinculin, zyxin, β-actin, α-tubulin, and other models.

TagRFP use for protein labeling in mammalian cells. Microscopic images of HeLa cells transiently transfected with TagRFP-tagged fusions. Images were kindly provided by Michael W. Davidson (Florida State University).

TagRFP suitability to generate stably transfected cells has been proven by Marinpharm company. Cell lines expressing TagRFP fusions are commercially available.

		Stably transfected cell lines expressing TagRFP fusions. U-205 mammalian cell lines expressing (A) TagRFP fusion with α-tubulin and (B) TagRFP fusion with β-actin. Images were kindly provided by Dr. Christian Petzelt (Marinpharm).

TagRFP can be used in multicolor labeling applications with other fluorescent proteins of cyan, green, yellow, and far-red colors.

		TagRFP use in multicolor labeling of mammalian cells. (A) TagCFP-tagged β-actin (cyan), TagYFP-tagged α-tubulin (yellow), TagFP635-H2B fusion (red), and Golgi-targeted TagRFP (violet); (B) mitochondria-targeted TagCFP (cyan), Dendra2-tagged vimentin (green), and TagRFP-tagged β-actin (red). Image (A) was kindly provided by Michael W. Davidson (Florida State University).